|
KMID : 1094720150200030488
|
|
Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 3 p.488 ~ p.497
|
|
|
Three-dimensional magnetic cell array for evaluation of anti-proliferative effects of chemo-thermo treatment on cancer spheroids
|
|
Yamamoto Shuhei
Okochi Mina
Jimbow Kowichi
Honda Hiroyuki
|
|
|
Abstract
|
|
|
|
Three-dimensional (3D) cell culture arrays of melanoma cell spheroids were assembled to evaluate the combined effect of a melanogenesis-targeting drug, N-propionyl-4-cysteaminylphenol (NPrCAP), and heat treatment. An array-like multicellular pattern of mouse melanoma B16F1 cells in a collagen gel was established by magnetic cell labeling using a pin-holder device to exert a magnetic force. The cellular spheroids were exposed to NPrCAP and heat (42°C for 1 h) as a model of anti-cancer treatment. As a result, melanogenesis of B16F1 cells was 29-fold higher in this 3D array than in conventional two-dimensional (2D) monolayer cultures. Because the spheroid size was linearly correlated with the cell number within a spheroid, the antiproliferative effect could be evaluated in a non-destructive manner. Moreover, the half-maximal inhibitory concentration of NPrCAP coupled with heat treatment calculated from the spheroid size was 2-fold higher in the 3D array (0.30mM) than in 2D culture (0.15 mM). These results indicate that spheroid formation decreases the chemosensitivity of cancer cells, and this model would be suitable as a susceptibility assay for melanogenesis-targeting drugs. Therefore, this 3D culture model provides a better screening format to evaluate drug and physical treatments for cancer therapy than 2D formats.
|
|
|
KEYWORD
|
|
|
melanoma, Spheroid, NPrCAP, hyperthermia, 3D cell culture, cell patterning
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|